Identification and characterization of human CD34+ and CD34dim/- neutrophil-committed progenitors (NCPs) [scRNA-seq]

In this study we report the identification of a unipotent human neutrophil-committed progenitors (NCPs) within bone marrow (BM) CD34+ and CD34dim/- cells. We show that NCPs can be either CD45RA+ or CD45RA-. By scRNA-seq experiments, we could uncover that NCPs actually consist of four cell clusters (c1-c4), substantially matching with the phenotypic identification of NCPs based on CD34, but not CD45RA, expression. c1-c4 cells were found to be at different maturation levels, aligned along two developmental neutrophil trajectories, as well as characterized by specific gene profiles. Examination by single cell RNA-seq of four BM neutrophil progenitors (CD34+CD45RA- NCP1, CD34+CD45RA+ NCP2, CD34dim/-CD45RA+ NCP3 and CD34dim/-CD45RA- NCP4) and the common monocyte progenitor (cMoP)