Expression Profile Analysis of Long non-coding RNAs in Mouse Liver

In the present study, the lncRNA expression profile of mouse fetal and adult liver was analyzed by ArrayStar LncRNA Array 3.0. Differentially expressed LncRNAs with statistical significance were identified through Volcano Plot filtering between two groups. Pathway analysis and GO analysis were applied to determine the roles of these differentially expressed lncRNAs played in these biological pathways or GO terms. Finally, Hierarchical Clustering was performed to show the distinguishable LncRNAs expression pattern among samples. The total RNA from C57/BL6 mouse fetal (embryo-E18) and adult (2-months old) liver were extracted and RNA integrity was assessed by standard denaturing agarose gel electrophoresis and Bioanalyzer. For lncRNA array analysis, ArrayStar Mouse LncRNA Microarray V3.0 was employed. Three independent experiments were performed at each condition (liver-E18 vs. liver-2months). To identify differentially expressed LncRNAs with statistical significance, we performed a Volcano Plot filtering between the two groups (Fold Change >= 2.0, P-value <= 0.05).本研究采用ArrayStar LncRNA芯片3.0对小鼠胎肝与成年肝脏的长链非编码RNA（long non-coding RNA, lncRNA）表达谱进行分析。通过火山图（Volcano Plot）筛选两组间具有统计学意义的差异表达长链非编码RNA；随后采用通路分析与基因本体论（Gene Ontology, GO）分析，明确上述差异表达长链非编码RNA在相关生物学通路及基因本体论条目中的生物学功能；最终通过层级聚类分析展示不同样本间长链非编码RNA的特征性表达模式。本研究提取了C57/BL6小鼠胎龄E18胚胎期以及2月龄成年小鼠的肝脏总RNA，并通过标准变性琼脂糖凝胶电泳与生物分析仪（Bioanalyzer）评估RNA完整性。针对长链非编码RNA芯片分析，本研究采用ArrayStar小鼠长链非编码RNA芯片V3.0。每组实验条件（即E18胎肝与2月龄成肝）均开展三次独立重复实验。为筛选得到具有统计学意义的差异表达长链非编码RNA，本研究对两组样本进行火山图筛选，筛选阈值为差异倍数（Fold Change）≥2.0且P值≤0.05。