Foodborne Salmonella enterica Isolates from Russia

Salmonella enterica is an important foodborne pathogen worldwide. Ciprofloxacin and extended-spectrum cephalosporins are the common first-line antimicrobial drugs for the treatment of salmonellosis. Antimicrobial resistance genes for which are mostly transferred through plasmids. Thus, the goal of this work was to perform genomic analysis of foodborne S.enterica plasmids obtained in Russia based on whole-genome sequencing.A total of 11 multi-drug resistant samples isolated in 2021 from 8 regions of Russia were selected by resistance to ciprofloxacin- and third-generation cephalosporins (CIP-3rd) in the current study. Whole genome sequencing by Illumina was obtained for all isolates to determine that the samples belong to 5 different sequence types (ST32, ST469, ST11, ST142, and ST548) which had different profiles of AMR and virulence genes. We have performed short- and long read sequencing of four representative S.enterica isolates showed that they carried pES-like megaplasmids of 202-280 kb length harboring extended-spectrum beta-lactamases genes, genes for resistance to fluoroquinolones, tetracycline and aminoglycosides as well as some virulence determinants.