Vibrio Cholerae CRISPR-cas PAM requirements
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Plasmids were constructed harboring protospacers that are perfect targets for spacers #4 and #21 of the array, but contain NNN (all possible PAM combinations) immediately...
本研究构建了携带有前间隔序列(protospacer)的质粒,该前间隔序列可作为CRISPR阵列中第4号与第21号间隔序列(spacer)的完美靶标,且紧邻其序列包含NNN序列(即所有可能的原间隔序列毗邻基序(PAM)组合)……



