Next Generation Sequencing Facilitates Quantitative Analysis of the effects of CB2 on lung ILC2 Transcriptomes. Next Generation Sequencing Facilitates Quantitative Analysis of the effects of CB2 on lung ILC2 Transcriptomes

CB2 signaling supports secretion of ILC2 effector cytokines ex vivo. Increased ILC2 activation leads to the development of airway hyperreactiviy and lung inflammation. Targeting CB2 signaling may therefore represent a novel therapeutic target to treat asthma. Overall design: mRNA profiles of sorted mouse lung ILC2s from either WT or CB2 KO mcie were generated by deep sequencing, in triplicate, using a NextSeq 500 (Illumina) system.