Whole genome sequencing of Arabidopsis anueploids. Arabidopsis thaliana

Trisomics of each of the five Arabidopsis thaliana chromosomes were screened out microscopically at the seedling stage from a population of selfed progeny of a triploid parent using fluorescent-tags on specific chromosomes as markers. The triploid parents are obtained as progeny from a cross between a diploid and a tetraploid. The trisomics were grown in soil and the number of chromosomes was confirmed by counting metaphase chromosomes prepared from flower pistils and by comparative genome hybridization.