The SWI/SNF ATPase BRG1 facilitates multiple pro-tumorigenic gene expression programs in SMARCB1-deficient cancer cells

Here, we expose the connection between rSWI/SNF and oncogenic processes using a well-characterized chemical degrader to deplete the SWI/SNF ATPase, BRG1. Using a combination of gene expression and chromatin accessibility assays we show that rSWI/SNF complexes facilitate MYC target gene expression. We also find that rSWI/SNF maintains open chromatin at sites associated with hallmark cancer genes linked to the AP-1 transcription factor, suggesting that AP-1 may drive oncogenesis in MRT. Overall design: We used a well-characterized chemical degrader targeted against the SWI/SNF ATPase, BRG1 to deplete BRG1 in the G401 MRT cell line. For RNA-seq and ATAC-seq, G401 cells were treated for 24 hours with 250 nM ACBI1 or matched DMSO control.