Gene Expression Profiling in progesterone-induced HGSC development.

Here we show that ovarian progesterone is a crucial endogenous factor inducing metastatic ovarian cancer in a mouse model of high-grade serous carcinoma (HGSC). To examine the molecular signaling pathways underlying progesterone (P4)-induced HGSC development, we performed gene-expression profiling using RNA sequencing. mRNA profiles of wild type (Ovid) mice, DKO (Dicer Pten conditional KO) mice and bilateral ovariectomy DKO mice, and bilateral ovariectomy DKO mice with treated P4 were generated by RNA sequencing, in triplicate, using Illumina NextSeq500.