Cell-type specific transcriptional profiling of glucocorticoid receptor-dependent changes in the nucleus accumbens

We have aimed to analyze dexamethasone-induced transcriptional profiling in astrocytes and neurons of the mouse nucleus accumbens (NAc). C57BL mice were injected with saline or dexamethasone (glucocorticoid receptor agonist, 4mg/kg), and gene expression was analyzed 4 hours later in the NAc tissue as well as magnetically sorted astrocytes and neurons. Samples from individual mice were hybridized to separate microarrays.