Deciphering the transcriptional changes in Escherichia coli strains C41(DE3) and C43(DE3) that makes them a superior choice for membrane protein production.

This study assessed the differential gene expression of C41(DE3) and C43(DE3) strains in comparison to their parental strain BL21(DE3), in the presence and absence of a prototypical protein expression vector with or without the inducer IPTG. Overall design: Part 1 of this two part study assessed the differential expression of E. coli strains C41(DE3) and C43(DE3) compared to BL21(DE3). assessed the differential expression of E. coli strains C41(DE3) and C43(DE3) compared to BL21(DE3) that had been transformed with a plasmid, pACYCDuet-1, and grown in the presence of the inducer IPTG. All samples were performed in triplicate. This part 2 of the study assessed the differential expression of E. coli strains C41(DE3) and C43(DE3) compared to BL21(DE3) that had been transformed with the plasmid, pACYCDuet-1, and grown in the presence of the inducer IPTG for two hours. All samples were performed in triplicate.