Single cell RNA-seq analysis of endothelial cells during diabetic atherosclerosis

Single-cell RNA sequencing (scRNA-seq) was performed on the CD31-mircrobeads enriched endothelial cells isolated from aorta and heart from eight-week-old, male Ldlr-/- mice fed standard chow (Chow) or diabetogenic high-fat diet with 0.15% cholesterol (DDC) diet for 12 weeks. This study report the changes of EC subpopulations, fractions, transcriptomic and metabolic profiles during diabetic atherosclerosis. Examination of the transcriptomic profiles of endothelial cells isolated from aorta and heart pooled from three Ldlr-/- mice fed standard chow (Chow) or diabetogenic high-fat diet with 0.15% cholesterol (DDC) diet for 12 weeks本研究对8周龄雄性低密度脂蛋白受体基因敲除（Ldlr-/-）小鼠的主动脉与心脏来源的、经CD31微球（CD31-microbeads）富集的内皮细胞开展单细胞RNA测序（single-cell RNA sequencing, scRNA-seq），受试小鼠分别饲喂普通饲料（Chow）或含0.15%胆固醇的致糖尿病高脂饮食（DDC饮食），干预时长为12周。本研究报道了糖尿病性动脉粥样硬化进程中内皮细胞亚群（EC subpopulations）、细胞占比、转录组谱及代谢组谱的变化。本研究同时对合并自3只饲喂普通饲料（Chow）或含0.15%胆固醇的致糖尿病高脂饮食（DDC饮食）12周的Ldlr-/-小鼠的主动脉与心脏来源内皮细胞的转录组特征进行了检测分析。