Gene expression profile of human ARPE-19 cells subjected to mitochondrial DNA double stranded breaks through expression of mitochondrial TALENs

Goal is to analyze the expression profile of cells shortly after induction of mitochondrial double stranded breaks. ARPE-19 cells are transfected with constructs coding for mitochondrial TALENs, either active or dead. These constructs target two different locations of mtDNA (ATP8 and Dloop). Gene expression is then compared between untransfected and transfected cells (either active or dead TALENs), or between active TALEN (cells with mitochondrial double stranded breaks) and dead TALEN (expressing the same protein without cutting the mitochondrial genome) Overall design: 11 total samples: 2 replicates for non transfected cells, 3 replicates for TALEN ATP8, 3 replicates for TALEN ATP8 dead, 3 replicates for TALEN Dloop.