Immunohistochemical localization of liver AQP9 in ob/ob mice.

Semi-thin liver sections submitted to immunoperoxidase as described in Materials and Methods. (A) Liver section from C57BL/6J control lean mouse. Immunolabeling (brown staining) is seen over the sinusoidal domain of the basolateral membrane of hepatocytes (arrows; inset). (B) Liver of C57BL/6J obese mice. Low AQP9 reactivity is present over the basolateral membrane of hepatocyte (arrows). Labeling is rather observed over the intracellular compartment (double arrowheads; inset). Large lipid droplets (ld), clear sign of macrovesicular steatosis, are seen in most hepatocytes. Considerable immunoreactivity is seen around the lipid droplets (single arrowheads) where organelles abound within the rim of cytoplasm surrounding the vesicle. ss, sinusoidal space. Bar, 30 µm.