Whole cortex and brain endothelial cell-specific RNA-seq of mice subjected to peripherally-induced inflammation

The blood-brain barrier is an interface for signal exchange between the brain and the periphery. We reasoned that brain endothelial cells (BECs) are ideally positioned to receive signals from the blood and transduce them into the brain via direct communication with astrocyte endfeet. In combination with the a method to define the in-vivo protein composition of astrocyte endfeet, we used mouse brain endothelial cell-specific RNA-seq to study BEC-endfeet interactions in response to peripheral inflammatory insult by LPS.