Differential time requirements for RR induction in HEp-2 cells treated with different CTPS1 and IMPDH2 inhibitors at 2 mM concentration.

1Ribavirin induced RR as early as 30 min and between 1 to 24 h, nearly all cells had RR (p<0.0001, 0 min vs all time points and 30 min vs 1 to 24 h). There is no statistical significance between 1 to 24 h after Bonferroni's adjustment;
2Induction of RR by DON was slower than Ribavirin (p<0.0001, 1, 2, and 3 h; ns, 30 min and 24 h) and the percentage of RR positive cells gradually increased after 1–3 h and was nearly all cells at 24 h.
3Acivicin had similar pattern to DON in which RR positive cells gradually increased after 1 h and nearly all cells at 24 h.
4Ribavirin vs DON and DON vs Acivicin, ns; Ribavirin vs Acivicin, p = 0.03;
5Ribavirin vs DON and Ribavirin vs Acivicin, p<0.0001; DON vs Acivicin, ns;
6Ribavirin vs DON, DON vs Acivicin, Ribavirin vs Acivicin, p<0.0001;
7ns between Ribavirin, DON, and Acivicin.