MicroRNA expression in the glomerulus of C57BL/6 mice and B6.MRLc1 glomerulonephritis mice

In isolated glomeruli by beads-perfusion methods, microRNA (miRNA) expression was analyzed in healthy C57BL/6 and B6.MRLc1 glomerulonephritis mice. The expression of 1,135 miRNAs was examined, and up-regulated or down-regulated miRNA was determined. These results provide a basical information of molecular pathology in glomerulonephritis. The glomeruli were collected from female C57BL/6 (n=3, 9-month-old, healthy control) and female B6.MRLc1 (n=3, 14-month-old, early stage of glomerulonephritis; n=3, 9-month-old, late stage of glomerulonephritis). The total RNA sample was purified, and total 1,135 microRNA expression was compared among healthy, early stage, and late stage.本研究通过微球灌注法分离肾小球，针对健康C57BL/6小鼠及B6.MRLc1肾小球肾炎小鼠的微小RNA（microRNA，miRNA）表达水平开展分析。本研究共检测了1135种微小RNA的表达情况，并鉴定得到上调及下调的差异表达微小RNA，研究结果可为肾小球肾炎的分子病理研究提供基础数据支撑。实验所用肾小球均采集自雌性小鼠：健康对照组为3只9月龄雌性C57BL/6小鼠；B6.MRLc1小鼠分为两个模型组，其中3只14月龄个体为肾小球肾炎早期模型，3只9月龄个体为肾小球肾炎晚期模型。实验中先对总RNA样本进行纯化，随后对比了健康对照组、疾病早期组与疾病晚期组之间1135种微小RNA的表达差异。