Single cell sequencing analysis of adipose tissue stroma from lean and obese female mice. Single cell sequencing analysis of adipose tissue stroma from lean and obese female mice

We report the single cell sequencing data from subcutaneous adipose tissue CD29+ stromal cells (mesenchymal stem cells). Subcutaneous adipose tissue was collected from a mouse model of obesity and breast cancer anti-estrogen therapy. Lean and obese mice were ovariectomized, supplemented with estradiol, then maintained on estradiol, given tamoxifen, or estradiol was withdrawn for 7 weeks. Overall design: Two to 3 mice per group (Low Fat or High Fat; E2, E2+Tamoxifen, E2 withdrawal) were used for tissue collection. Adipose was digested, stromal fraction was depleted of CD45+ and CD31+ cells, while CD29+ cells were retrieved by FACS. Approximately 10,000 cells per group of pooled samples (2-3 females pooled per diet and treatment group) were sequenced.