东北红豆杉枝叶黄酮类化合物的提取、纯化及生物活性研究
收藏国家林业和草原科学数据中心2022-11-16 更新2024-03-06 收录
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In this paper, the optimal operational and process parameters were determined for the enzymatic hydrolysis and ultrasound-assisted extraction (EHUE) method of flavonoids extracted from Taxus cuspidata branches and leaves (TCBL), and the biological activity of obtained flavonoids was evaluated. According to single factor and central composite design experiments, the optimum key experimental parameters for EHUE were that pectinase enzyme concentration was 0.10 mg center dot mL-1, enzymatic hydrolysis temperature was 48 degrees C, and enzymolysis time was 39 min. The yield of flavonoids from TCBL under the optimized conditions was 5.23% +/- 0.18%. Four purified flavonoid compounds from TCBL extract were identified as 1) (E)-1-methoxy-2-O-(pcoumaroyl)-myo-inositol, 2) catechin, 3) epicatechin, and 4) quercetin-3-Oglucoside.
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国家林业和草原科学数据中心
创建时间：
2022-11-16
