Gene expression profile in the tethered Nrf2-MafG (T-N2G) heterodimer-expressing small Maf-deficient cells [RNA-seq]

To elucidate the function of Nrf2-small Maf heterodimer, we constructed a tethered Nrf2-MafG (T-N2G) heterodimer using a flexible linker peptide and examined its function in the small Maf-deficient mouse embryonic fibroblast (MEF). Overall design: polyA RNA-seq was performed using RNA from Immortalized MafF-/-::MafG-/-::MafK-/- (F0G0K0) MEFs and T-N2G-expressing F0G0K0 MEFs, treated with 100µM Diethyl Maleate (DEM) or Vehcle for 12hr. sample title - condition - replicate (in the genes.read_group_tracking.txt) 2_Mock_Veh - Mock_Veh - 1 1_Mock_Veh - Mock_Veh - 0 3_Mock_Veh - Mock_Veh - 2 2_Mock_DEM - Mock_DEM - 1 1_Mock_DEM - Mock_DEM - 0 3_Mock_DEM - Mock_DEM - 2 3_T-N2G_Veh - N2_Veh - 2 1_T-N2G_Veh - N2_Veh - 0 2_T-N2G_Veh - N2_Veh - 1 1_T-N2G_DEM - N2_DEM - 0 2_T-N2G_DEM - N2_DEM - 1 3_T-N2G_DEM - N2_DEM - 2