Figure S1 - Involvement of the TNF and FasL Produced by CD11b Kupffer Cells/Macrophages in CCl4-Induced Acute Hepatic Injury

The recruitment of adoptive transferred F4/80+ cells into liver. Liver MNCs were isolated from CCl4 treated mice and stained with PE-Cy5 labeled anti-F4/80 antibody. F4/80+ and F4/80− cells were separated using anti-PE magnetic beads and MACS sorting device. Sorted F4/80+ cells labeled with PE-Cy5, and F4/80− cells without labelling, were adoptively transferred into normal mice. Then, liver MNCs obtained from each recipient mouse at 1.5 h after adoptive transfer were analyzed by flow cytometry without additional staining. PE-Cy5 (F4/80) positive cells were counted and depicted in red area and dots. Data are representative of three mice in each group, with similar results. (EPS)