five

IL-27 in vaccine elicited cellular immunity

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NIAID Data Ecosystem2026-04-25 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP139325
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5000 congenically marked (CD45.1) WT or IL-27R-/- OT1 T cells were transferred into WT B6 hosts (CD45.2). One day later, the mice were immunized with ovalbumin (100 µg), PolyIC (40 µg) and anti-CD40 antibody (FGK4.5, 40 µg). 3 days after immunizayion, spleens were harvested and the OT1 T cells isolated by flow sorting based on CD45.1 expression. Each sample is the result of pooling T cells from 3 individual mice. RNA was isolated from the sorted cells and RNAseq performed. Overall design: RNAseq of transferred WT and IL-27R-/- OT1 T cells isolated 3 days after combined polyIC/aCD40 immunization
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2020-04-17
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