Whole transcription data for mouse spermatogonial cells

Spermatogenesis is a multi-step yet tightly regulated developmental process to produce male gemetes for reproduction. In mouse spermatogenesis, a sub-group of type A spermatogonia (Spga) known as spermatogonial stem cells (SSCs) maintain their population by self-renewal, and differentiate through mitosis and meiosis to form tetraploid (4n) pacchytene spermatocytes (Spcy) and haploid (n) round spermatids (Sptd), which further differentiate into functional sperms. In this study, we selected three representative stages of mouse spermatogenesis, namely Spga, Spcy and Sptd, to study their transcriptomic charteristics using whole genome tiling microarray. Three biological replicates of each selected stage. Spga were isolated from 6-day-old Balb/c mice, Spcy and Sptd from 60-day-old Balb/c mice. Transcriptomic landscapes of these three cell types were analysed with whole genome tiling array.精子发生（spermatogenesis）是一类多步骤且受严格调控的发育过程，其功能为产生雄性生殖细胞以实现繁殖。在小鼠精子发生过程中，A型精原细胞（type A spermatogonia, Spga）中的亚群——精原干细胞（spermatogonial stem cells, SSCs）可通过自我更新维持种群规模，并经有丝分裂与减数分裂逐步分化，先后形成四倍体（tetraploid, 4n）粗线期精母细胞（pachytene spermatocytes, Spcy）与单倍体（haploid, n）圆形精子细胞（round spermatids, Sptd），后者可进一步分化为功能性成熟精子。本研究选取小鼠精子发生的三个代表性阶段：Spga、Spcy与Sptd，采用全基因组拼接微阵列（whole genome tiling microarray）分析其转录组特征。每个阶段均设置三次生物学重复，其中Spga分离自6日龄BALB/c小鼠，Spcy与Sptd则分离自60日龄BALB/c小鼠，最终通过全基因组拼接微阵列解析了这三种细胞类型的转录组全景。