Expression Data from CD4+CD25+Nrp1+ regulatory T cells of Foxp3creCREBfl/fl mice

Regulatory T cells (Tregs) are gatekeepers of immune homeostasis and characterized by expression of Foxp3, which maintains Treg identity. The transcriptional activator CREB critically stabilizes Foxp3 expression in vitro. Here we demonstrate that in mice with a Foxp3-specific knockout of CREB, Tregs show a reduced Foxp3 expression in vivo, but surprisingly enhanced expression of IL-13, IL-10, ST-2 and CREM. This rendered such Tregs highly suppressive in vitro and prevents disease activity in Th1 models like T cell mediated transfer colitis in an IL-10 dependent way. On the other hand, type 2 B cell responses were enhanced resulting in high IgE levels and susceptibility towards experimental asthma. Our data suggest that CREB expression in Tregs is important for the balance between Th1 and Th2 responses by regulating ST-2 and IL-10. We used microarrays to detail the global programme of gene expression in CD4+CD25+Nrp1+ T cells of Foxp3creCREBfl/fl mice Genome-wide transcriptome analyses for Foxp3creCREBfl/fl and WT CD4+CD25+Nrp1+ T cells were performed in independent triplicates using Gene Chip® MTA 1.0 st arrays