small RNA profiling of extracellular RNA from breast cancer cell lines [EV]

We performed small RNA sequencing of exosomes and extracellular vesicles collected from breast cancer cells as well as human mammary epithelial cells (HUMEC) in biological replicates (using Norgen's cell culture media exosme purification and RNA isoaltion kits). BRCA-lines_EV_smRNA: Eight breast cancer lines and human mammary epithelial cells were cultured in biological replicates. Exosomes/small extracellular vesicles (EV) were then collected from the conditioned media using the Cell Culture Media Exosome Purification Mini Kit (Norgen). Samples were then subjected to small RNA sequencing. The number of unique orphan non-coding RNAs were then recorded for each sample.