Single cell transcriptome mapping reveals cell type-specific effects on gene expression by acute delta9-tetrahydrocannabinol

The study identifies cell type specific differentially expressed genes in peripheral blood mononuclear cells affected by THC infusion in two human subjects. Individual gene expression gene analysis and gene set enrichment analysis show that THC affects gene expression in the functional domains of immune response and cell proliferation. One of THC receptor genes, CNR2, is highly expressed in B cells and THC perbute CNR2 co-expressed genes in B cells. The findings provide new insight of acute THC immunotonary effects. Overall design: A single low dose of THC infusion in two human subjects and detect differentially expressed genes between pre- and post-THC infusion.