miRNA expression profiles of chicken embryonic visceral tissue infected with F48E9 or La Sota

Three cDNA libraries of mixed visceral tissues from F48E9, La Sota, or uninfected chicken embryos were constructed, and small-RNA deep sequencing was conducted to detect the expression levels of small-RNAs. Intergroup comparisons were used to identify changes in miRNA expression caused by NDV infection. La Sota affected the expression of 61 miRNAs (36 upregulated and 25 downregulated) at 36 hpi, and F48E9 infection altered the expression levels of 66 miRNAs (33 upregulated and 31 downregulated). Using high-throughput sequencing, revealed miRNA expression differences of SPF chick embryos infected different virulence NDVs.