TNFR2 HnRNPK-YAP Signaling Axis Promotes Primary Liver Cancer Development in Hepatic Progenitor Cells

We performed single-cell RNA sequencing (scRNA-seq) analysis using clinical samples of PLC patients. We first generated scRNA-seq profiles of freshly isolated PLC from patients with 10x Genomics. A total of 25,189 single-cell transcriptomes from tumors of two patients were obtained after initial quality controls. We performed scRNA-seq analysis with tumor and adjacent tissue of two PLC patients. Then, we performed t-distributed stochastic neighbor embedding (t-SNE) analysis of all cells, which were grouped mainly according to their cell types as annotated based on known cell lineage-specific marker genes.We further confidently discriminated malignant cells by inferring large-scale chromosomal copy-number variations (CNVs) based on transcriptomes.