Hoxb1 mutant mRNA levels in rhombomere 4 at E10.5

Hoxb1 is required for proper specification of rhombomere 4 and the facial motor neurons. This study analyzed gene expression in the corresponding hindbrain segment of E10.5 mutant embryos. Several genetic pathways were found altered, including transcription factors such as Phox2b, Gata3, Nkx2-2 and Nkx6-1. Keywords: hindbrain development, rhombomere 4, Hoxb1 Each of the three mutant and control samples was an independent biological replicate. Pools of r4 segments from multiple embryos were snap frozen on dry ice and stored at -80ºC. Total RNA was isolated, processed with standard Affymetrix protocols and hybridized to GeneChip Mouse Expression Sets 430A.同源框基因B1（Hoxb1）对于菱脑节4（rhombomere 4）和面运动神经元的正常特化至关重要。本研究针对胚胎发育第10.5天（E10.5）的突变小鼠胚胎的对应后脑节段开展了基因表达分析。经分析发现多条遗传通路出现异常改变，其中包括Phox2b、Gata3、Nkx2-2及Nkx6-1等转录因子。 关键词：后脑发育、菱脑节4、Hoxb1 三份突变样本与对照样本均为独立生物学重复。从多枚胚胎中获取的r4节段混合样本经干冰快速冷冻后，于-80℃条件下保存。提取总RNA后，依照标准Affymetrix实验流程进行处理，并与GeneChip小鼠表达集430A（GeneChip Mouse Expression Sets 430A）进行杂交。