Comparing the miRNA transcriptome of FAPs cultured in basal medium (BM) and adipogenic differentiation medium (ADM)

FAPs isolated by FACS from wildtype mice were grown in culure and then placed in BM or ADM for 24 hours to assess the miRNA transcriptomic changes FACS isolated FAPs from wildtype (WT) mice were cultured in growth medium (GM) until ~80% confliuent. The cells were then switched to BM or ADM and maintained for 24 hours, at which time small RNAs were isolarted uisng the miRVana Kit. Small RNA libraries were then prepared with the Illumina TruSeq Small RNA Sample Prep kit. An additional size selection for barcoded miRNAs at band size of 147bp was completed on a TBE PAGE gel. Libraries were sequenced on an Illumina MiSeq system at the Stanford Functional Genomics Center (SFGC).