Additional file 1 of Elucidating the roles of three β-glucuronosyltransferases (GLCATs) acting on arabinogalactan-proteins using a CRISPR-Cas9 multiplexing approach in Arabidopsis

Additional file 1: Five supplemental tables, three supplemental figures and their corresponding legends. Table S1. List of guide RNA sequences and their target genes. Table S2. List of primers used to assemble three gRNAs (A1, B1, and C1) into pHEE401E binary vector. Table S3. List of primers used to assemble four gRNAs (A2, B1, C2, and C3) into pHEE401E binary vector. Table S4. List of primers for sequencing off-targets. Table S5. List of primers used for qRT-PCR. Figure S1. Amino acid sequence alignment of GLCAT14A, GLCAT14B, and GLCAT14C. Figure S2. Expression profiles of GLCAT14A, GLCAT14B, and GLCAT14C obtained from the Klepikova Arabidopsis Atlas eFP Browser of the Bio-Analytic Resource for Plant Biology (BAR) [18]. The link for the expression profile of GLCAT14A can be found at http://bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi?primaryGene=AT5G39990&dataSource=Klepikova_Atlas&modeInput=Absolute; The link for the expression profile of GLCAT14B can be found at http://bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi?primaryGene=AT5G15050&dataSource=Klepikova_Atlas&modeInput=Absolute; The link for the expression profile of GLCAT14C can be found at http://bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi?primaryGene=AT2G37585&dataSource=Klepikova_Atlas&modeInput=Absolute. Figure S3. Expression patterns of GLCAT14A, GLCAT14B, and GLCAT14C during seed development obtained from the Arabidopsis eFP Browser of the Bio-Analytic Resource for Plant Biology (BAR) (bar.utoronto.ca). The link for the expression profile of GLCAT14A during seed development can be found at http://bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi?dataSource=Seed&modeInput=Absolute&primaryGene=At5g39990&secondaryGene=At3g27340&override=None&threshold=219.05&modeMask_low=None&modeMask_stddev=None; The link for the expression profile of GLCAT14B during seed development can be found at http://bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi?dataSource=Seed&modeInput=Absolute&primaryGene=AT5G15050&secondaryGene=At3g27340&override=&threshold=17.75&modeMask_low=None&modeMask_stddev=None&gene_alias1=GlcAT14B&gene_alias2=; The link for the expression profile of GLCAT14C during seed development can be found at http://bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi?dataSource=Seed&modeInput=Absolute&primaryGene=AT2G37585&secondaryGene=At3g27340&override=&threshold=18.13&modeMask_low=None&modeMask_stddev=None&gene_alias1=GlcAT14C&gene_alias2=.