The codon-optimized zAncBE4max construct contains the Uracil glycosylase inhibitor(UGI) and Deaminase (rAPOBEC1)-coupled Cas9a D10A nicknase, and NLS to enabe C-to-T base editing in the whole genome o
Genomic DNA was extracted from the separate body parts of Danio rerio adult and embryo using the standard protocol. PCR was performed using the primers: Term1: AAAAAAAAAAAAAAAACCTAACCCTAACCC Term2: AA
