Next Generation Sequencing of Wild-Type FVB/NJ Mouse Cardiac Small RNA

Purpose: Next-generation sequencing (NGS) provides for quantitation of RNA abundances and comparison of RNA abundances within tissues and cells in a manner not possible with previous microarray technologies. We have made widespread use of Illumina sequencing technologies for RNA quantitation in several publications involving mouse hearts, dating from 2010, and wish to share both high-quality raw sequencing data and data processed to quantitate mRNA abundances from wild-type mice, male and female, at a variety of ages. These data will provide a resource for investigators using microarrays to understand the concentration of transcripts of interest relative to other cardiac RNAs, and will permit deeper interpretation of previous microarray studies. 39 cardiac small RNA (microRNA) profiles of 4- to 16 week-old FVB/NJ wild type (WT) mice were generated on Illumina HiSeq 2000 instruments.研究目的：下一代测序（Next-generation Sequencing, NGS）能够实现RNA丰度定量以及组织、细胞内RNA丰度的比较，这是以往微阵列技术（microarray technologies）无法实现的。本团队自2010年起，在多项针对小鼠心脏的研究中广泛采用Illumina测序技术开展RNA定量分析，现希望共享高质量的原始测序数据，以及经处理后得到的不同性别、不同周龄野生型（Wild Type, WT）小鼠的mRNA丰度定量数据。此类数据可为使用微阵列技术的研究人员提供研究资源，帮助其明确目标转录本相较于其他心脏RNA的表达丰度，同时可助力对既往微阵列研究进行更深入的解读。本数据集包含39份4至16周龄FVB/NJ野生型（WT）小鼠的心脏小RNA（microRNA）表达谱，所有数据均通过Illumina HiSeq 2000测序仪生成。