Primer sequence of genes.

The present study evaluated the effects of curcumin on growth, immune and antioxidant response in tilapia (Oreochromis niloticus). An optimum dose of curcumin was investigated by feeding four different levels of this compound in combination with three different regimes of stocking density (12 treatments). Fish were reared at three densities; low density (LD = 1.50 kg/m3), medium density (MD = 3.00 kg/m3), and high density (HD = 4.50 kg/m3). Each treatment was fed with four different levels of dietary supplementation of curcumin (C0 = 0 mg/kg, C1 = 50 mg/kg, C2 = 100 mg/kg, and C3 = 150 mg/kg) for 60 days. Each treatment has three replicates (n = 50/replicate in LD, 100/replicate in MD, 150/ replicate in HD). Although better growth was observed in MD, however treatments at all densities fed with C1 diet showed improved growth as compared to other diets. Chemical composition of fish and activity of amylase, lipase and protease in all treatments were noted to be similar. Levels of antioxidant enzymes (catalase, superoxide dismutase and glutathione peroxidase) and cortisol in MD and HD treatments were similar to those in LD treatment. However, fish fed with C1 diet in each density treatment showed the lowest values of antioxidant enzymes. Similarly, the levels of malondialdehyde were noted to be similar in MD and HD treatments as compared to that in LD. Its levels were lower in fish fed with C1 and C3 diets in all density treatments. Expression of pro-opiomelanocortin-α (POMC-α), Somatostatins-1 (SST-1) and Interleukin 1-β (IL-1β) did not increase in MD and HD treatments in response to high stocking density when compared with LD treatment. The lowest levels of these genes were noted in fish fed with C2 and C3 diets in all treatments. In conclusion, supplementation of curcumin in diet of tilapia improved growth and antioxidant response in tilapia. optimum dose of curcumin for tilapia culture is 50 mg/kg at the density of 3.00 kg/m3which might be further investigated for intensive culture.