five

TGF-ß treatment of human melanoma cell lines

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NIAID Data Ecosystem2026-03-11 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE148767
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To analyse whether the activation of TGF-ß signalling (recombinant hTGF-ß, 5 ng/mL; PeproTech) is able to invoke a full phenotype- switch, we performed RNA sequencing of melanoma cultures from two independent melanoma cell lines at two different time point after TGF-β1 (5 ng/ml, preprotech). Control and treated replicates were processed using using TRIsure lysis buffer (BioLine, BIO-38032) according to the directives of the manufacturer. RNA quality was assessed using the 2100 Bioanalyzer (Agilent) prior to expression profiling.
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2020-07-19
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