Development of gene expression signatures for experimental lingual carcinogenesis

To identification of gene expression profiling during lingual tumorigenesis, tongue cancer was induced by oral delivery of 4-nitroquinoline-1-oxide (4NQO) at a dose of 50mg/L for 16weeks in C57BL/6J female mice. Nine samples of lingual mucosa from 0th (encoded as C in experiment), 12th ((encoded as M in experiment) and 28th ((encoded as E in experiment) week which were supposed as normality, early stage and cancer during lingual tumorigenesis were harvested for microarray. 2339, 2539 and 2482 genes were significantly varied between M vs. C, E vs. M and E vs. C respectively. Chemical carcinogen 4-nitroquinoline-1-oxide induced gene expression in C57BL/6J female mice was measured at 0th (C), 12th (M) and 28th (E) week after oral delivery of 4-nitroquinoline-1-oxide (4NQO) at a dose of 50mg/L. Three independent experiments were performed at each time (0th ,12th ,28th week) using lingual mucosa.