Next Generation Sequencing Quantitative Analysis of altered expression of genes in BCa cells-induced PDGFRα+ITGA11+ CAFs and other CAFs

The cellular diversity of stromal compartment within the tumor microenvironment might change dynamically as tumors evolve that allowing distant dissemination of tumor cells and informing the treatment options. We used single cell RNA sequencing (scRNA-seq) to provide a detailed insight of cellular components feature in bladder cancer and identify that PDGFRα+ITGA11+ CAFs were essential for lymphatic metastasis of bladder cancer. To identify molecular mechanism of PDGFRα+ITGA11+ CAFs formation, next generation sequencing (NSG) was performed to explore the upregulated genes in bladder cancer-induced PDGFRα+ITGA11+ CAFs. To detect the precise mechanism of bladder cancer in inducing the formation of PDGFRα+ITGA11+ CAFs, the total RNAs of bladder cancer-induced PDGFRα+ITGA11+ CAFs and control CAFs were extracted and the next generation sequencing was conducted to explore the expressing profile difference between different groups. The results were obtained from three independent experiments.