Amplicon sequence data of IgM knockout Atlantic salmon

The dataset contains sequence data of gene-edited Atlantic salmon generated by high-throughput sequencing on the Illumina MiSeq platform. The salmon have been genetically modified by CRISPR/Cas9 knockout of IgM (two genes in salmon, A and B). Genomic DNA has been extracted from either fin tissue or leukocytes (white blood cells). Gene-specific primers have been designed to amplify both IgM genes (~400 bp) using PCR, and the amplicons have been barcoded via a second PCR. After cleanup the samples have been sequenced on the Illumina MiSeq platform using 300 bp paired-end reads.IgM crispants, named IgM_1 to IgM_20 (n=20), together with control fish, named Alb_1, Alb_10, WT_1 and WT_10 (n=4), were sequenced with the following distribution: DNA was obtained from fin of IgM_1 to IgM_ 20 (n=20) and control fish Alb_1 + WT_1 (n=2), and from cells of IgM_15 to IgM_20 (n=6) and control fish Alb_10 and WT_10 (n=2).How to orientate the dataset:Each folder is labeled "TargetGene_WellNumber_SampleNumber_L001_RX_001.fastq.gz"The TargetGene corresponds to either IgM-A or IgM-BWellNumber corresponds to which barcode was used and thus SampleIDRX corresponds to read 1 or read 2 (forward or reverse read)Example: "IgM-A_H2_S16_L001_R1_001." is fastq data obtained from the gene IgM-A and from well H2 (see list below for what sample this is).SampleBarcode(well)IgM_1(fin)H12 / A6IgM_2(fin)B1 / B6IgM_3(fin)C1 / C6IgM_4(fin)D1 / D6IgM_5(fin)E1 / E6IgM_6(fin)F1 / F6IgM_7(fin)G1 / G6IgM_8(fin)H1 / H6IgM_9(fin)A2 / A7IgM_10(fin)B2 / B7IgM_11(fin)C2 / C7IgM_12(fin)D2 / D7IgM_13(fin)E2 / E7IgM_14(fin)F2 / F7IgM_15(fin) G2 / G7IgM_16(fin)H2 / H7IgM_17(fin)A3 / A8IgM_18(fin)B3 / B8IgM_19(fin)C3 / C8IgM_20(fin)D3 / D8Alb_1(fin)E3 / E8WT_1(fin)F3 / F8Alb_10(cells)G3 / G8WT_10(cells)H3 / H8IgM_15(cells)A4 / A9IgM_16(cells)B4 / B9IgM_17(cells)C4 / C9IgM_18(cells)D4 / D9IgM_19(cells)E4 / E9IgM_20(cells)F4 / F9<br><br><br>