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VECTOR FOR MODIFYING THE EXPRESSION OF GENES OF INTEREST

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国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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The present invention relates to a promoter sequence specific for expression of genes of interest in fruit endosperm. The invention further describes DNA constructs that contain the promoter, as well as the method using such constructs to produce transgenic plants, plant cells or protoplasts. The expression of the transgene only in the organ of interest enables accumulation of the exogenous transcript only in the fruit and constitutes an advantage over the constitutive expression, since it favors implementation of strategies that aim at obtaining cultivars having higher aggregate value like: adaptation to environmental stress, tolerance to pathogens, pests and agricultural defensives, increase in the nutritional and therapeutic value and a new alternative to systems of expression in plant organism, generating more competitive cultivars at lower costs. Besides, the present invention also presents a sequence with constitutive expression, providing an alternative to the implementation of expression system where the constitutive expression is required. The invention has the objective of increasing the economic, social and, environmental and biosafety benefits associated to the genetic transformation. The present invention relates to a specific promoter sequence for the expression of genes of interest in fruit endosperm. The invention also describes DNA constructs that contain the promoter, as well as the method that uses these constructs to produce plants, plant cells or transgenic protoplasts. The expression of the transgene exclusively in the organ of interest allows the exogenous transcript to accumulate only in the fruit and is advantageous over constitutive expression, since it makes it easier to implement strategies that aim at obtaining cultivars with higher added value, such as: adaptation to environmental stresses, resistance to pathogens, pests and agrochemicals, increase in nutritional and therapeutic value, and a new alternative for expression systems in plant organisms to generate more competitive cultivars at lower costs. Moreover, the present invention also describes a sequence with constitutive expression, which offers an alternative for implementing expression systems when constitutive expression is required. The objective of the invention is to increase the economic, social and environmental benefits, as well as the biosafety of genetic engineering.
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国家林业和草原科学数据中心
创建时间:
2023-02-12
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