Transcriptomics and metabolomics reveal the changes of regulatory mechanisms of osteosarcoma under different culture methods in vitro

The purpose of this study was to reveal the differences in transcriptional and metabolic levels between osteosarcoma 2D cells, 3D cells, 3D cell printed tissue, isolated tissue, and transplanted tumor in vivo Overall design: Comparative gene expression profiling analysis of RNA-seq data for osteosarcoma Soas-2 2D cells, 3D cells, 3D cell printed tissue, isolated tissue, and transplanted tumor