Glycation-mediated tissue-level remodeling of brain meningeal membrane by aging

We performed bulk transcriptomic analysis to examine how glycation-modified collagen further impacts the function of meningeal fibroblasts. We preprocessed raw data to align bulk RNA-seq using STAR (detailed in Experimental Section) and identified differentially expressed genes (DEGs), especially ECM-related gene sets. Overall design: One biological sample of human leptomeninges-derived fibroblast cells was analyzed for each of 2 conditions: VC – vitrified collagen membrane without glycation GVC – vitrified collagen membrane with glycation