Computer simulations of the mutation frequency and percentage of inhibition of viral infectious progeny production, as a function of the multiplicity of infection (MOI) and of the cis- trans interactions.

aThe mutation frequency is the average of ten simulations and is expressed as the number of mutations per genome (mut/genome) or mutations per nucleotide (mut/nt).bThe inhibition of infectious virus progeny production is calculated according to , where, V(U = 0.15) is the number of viable progeny viruses (the equivalent of viral titer) when the mutation rate is U = 0.15 mutations/genome/replication round, and V(U) is the number of viable progeny viruses when the mutation rate is U = 4.5 or U = 7.5. Results are the average of ten simulations.