Mitigating antagonism between transcription and proliferation allows near-deterministic cellular reprogramming (Single Cell)

RNA sequencing analysis of Hb9::GFP mouse embryonic fibroblasts infected with control or reprogramming transcription factors at 4 dpi, and Hb9::GFP+ mouse induced motor neurons generated using transcription factor overexpression. The goal of this study is to examine the transcriptional path of reprogramming cells to identify molecular mechanisms and populations of highly efficient reprogramming. Transcriptional profiling of cells during the process of direct reprogramming of mouse embryonic fibroblasts to induced motor neurons (iMNs) and at the terminal stage at 17 dpi. Samples include Hb9::GFP+ sorted iMNs generated with six factors (6F), 6FDD (e.g. 6F with p53DD), or 6FDDRR (6F with p53DD, hRASG12V, RepSox) as well as MEFs and samples at 4 dpi in conversion for 6F and 6FDDRR for both bulk and CFSE-low populations. The 6F are Ascl1, Brn2, Myt1l, Ngn2, Isl1, Lhx3.