Open Science - Metamorphosis Movies S2

Time-lapse X-ray video of pupation in six specimens of Calliphora vicina, beginning at 28 hours after pupariation (00:00, h:min). Images are displayed at one frame per second, each image captured at intervals of two-minutes (i.e. 120 x natural speed). The puparia on the top row (A-C) were imaged with the dorsal surface uppermost (specimen B inclined slightly to the left) while the puparia on the bottom row (D-F) were imaged with their left side uppermost. All puparia have their head at the top of the video. See text and Figure 1 for further explanation. Note that the gas bubble in specimen D has already started to move from the pupa at the start of the video and that the process starts next in specimen E then sequentially in specimens B, C, A and finally in F.