PMACS-95

<b>Project title</b>: PMACS-95<b>Grant Information:</b> AHA Grant 9TPA34910007, Regulatory role of pericardial macrophages in myocardial infarction<b>Relevant Citations:</b><b> </b><b>https://doi.org/10.1016/j.isci.2024.110244</b><b>Project Description:</b>· Animal Model: N/A – In Vitro Experiment using Mouse Cells· Animal Age: 8-13 Weeks· Animal Sex: Male· Animal Strain: C57B6/J· Number: 5 Mice· Groups:o Fibroblasts Cultured Alone§ With and Without 100ng/mL LPS Stimulationo Bone marrow derived macrophages alone§ With and Without 100ng/mL LPS Stimulationo Bone Marrow Derived Macrophages co-cultured with Fibroblasts§ With and Without 100ng/mL LPS Stimulationo Bone Marrow Derived Macrophages Cultured on Transwell plate with Fibroblasts on Bottom Plate§ With and Without 100ng/mL LPS Stimulationo Peritoneal Macrophages alone§ With and Without 100ng/mL LPS Stimulationo Peritoneal Macrophages co-cultured with Fibroblasts§ With and Without 100ng/mL LPS Stimulationo Peritoneal Macrophages cultured on Transwell Plate with Fibroblasts on Bottom Plate§ With and Without 100ng/mL LPS Stimulation· Experimental Conditions: Peritoneal fibroblasts, bone marrow derived macrophages, and peritoneal macrophages were all harvested from B6 mice. Fibroblasts were plated at 20,000 cells/well in AMCF media. After 24 hours, macrophages were plated at 30,000 cells/well either alone, on top of fibroblasts, or with transwell in BMDM media. 24-hours later, media removed and replaced with AMCF containing 100 ng/mL LPS. Cells were harvested after 16 hours and examined via qPCR.<b>Licenses: </b>N/A<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>