Expression profiling of mouse primary prostate cells from WT mice

By single-cell expression profiling and validation, here we report that both basal and luminal-localized cells, particularly the latter, are molecularly heterogeneous. We validate LY6D as a marker linking CR luminal cells to luminal progenitors. Primary prostate cells were isolated and luminal prostate cells were FACS-sorted by LY6D+/- SCA1High/Int/low from the lineage (CD31, CD45, Ter119) negative fraction. Total RNA was extracted from two biological replica test. Lexogen Quantseq RNA Sample Prep Kit was used with 10-20 ng of total RNA for the construction of sequencing libraries.