Mycobacterium tuberculosis variant bovis BCG strain:Pasteur 1721 Genome sequencing

The M. bovis BCG Pasteur strain 1721, used to create the sapM::Tn BCG mutant strain (Festjens et al, EMBO Mol Med 2011; Vandewalle et al, Nat Commun 2015), differs from the 1173P2 Pasteur vaccine strain by a K43R point mutation in the rpsL gene, conferring streptomycine resistance (Master et al, Cell Host Microbe 2008), which is useful to avoid contaminations during the lengthy and involved procedures in ordered transposon mutant library production. To investigate whether there are any additional variants between the WT BCG Pasteur and the sapM::Tn BCG mutant, we performed a whole-genome resequencing analysis on both strains (paired end 2 x 150 bp), mapping to the M. bovis BCG Pasteur 1173P reference genome, resulting in ± 80x average coverage of mapped reads.