Mus musculus breed:C57BL/6 Transcriptome or Gene expression

The accumulation of tumor-associated macrophages (TAMs) in the tumor microenvironment (TME) plays a crucial role in promoting tumor immune escape and immunotherapy tolerance. Sphingosine kinase 1 (SPHK1) is a key enzyme that mediates the conversion of sphingosine to sphingosine-1-phosphate (S1P), and tumor-intrinsic SPHK1 participates in the development of various cancers. However, the effects of SPHK1 within TAMs on tumor immune evasion during tumor progression remain elusive. Here, to determine the role of the SPHK1-S1P signaling in macrophage functions, we extracted bone marrow cells from 6-week-old male C57BL/6 mice and differentiated them into bone marrow-derived macrophages (BMDMs) with M-CSF treatment. The BMDMs were stimulated with S1P or bovine serum albumin (BSA) in serum-free medium for 12 hours. Subsequently, the cells were subjected to RNA sequencing to analyze the gene expression profiles.