Single-cell RNA-seq of isolated non-parenchymal cells from imiquimod-induced psoriasis mouse model.

We applied 10x Genomics single-cell RNA sequencing to profile non-parenchymal liver cells (NPCs) in a psoriasis-like mouse model. The study focuses on immune and liver sinusoidal endothelial cell (LSEC) alterations along the skin–liver axis to elucidate mechanisms driving comorbid liver disease in psoriasis. Given that 30–50% of psoriasis patients develop liver involvement, this approach aims to identify maladaptive cellular crosstalk and potential therapeutic targets. Overall design: 10 weeks old female C57BL/6J wild-type mice received topical imiquimod (5%, Aldara) on the shaved upper back for five consecutive days to induce psoriasis-like skin inflammation. Control mice were treated under the same conditions using petroleum jelly/Vaseline®.