RNASeq analysis of endothelial cells isolated from B16F0 tumours treated with IgG/ 15C4.

LRG1 is a novel proangiogenic and profibrotic molecule that is upregulated in pathologies characterised by abnormal angiogenesis such as AMD and cancer. Blocking LRG1 by genetic models or by our in-house developed function blocking antibody leads to vascular normalsation. In this study, we have treated B16F0 melanoma tumours with 15C4, our function blocking antibody, or with IgG as a control. The tumours were collected at day 17 and the endothelial cell population was isolated by FACS sorting the CD31 positive cells. RNA was isolated and used for the preparation of cDNA libraries. Expression profile of the endothelial cell population was assessed by Illumina sequencing. Differenital expression analysis was conducted using the DESeq2 package in R. Examination of the expression profile of the FACS-sorted endothelial population of the IgG-/15C4-treated B16F0 melanoma tumours.