SOX2 regulates foregut squamous epithelial homeostasis and is lost during Barrett’s esophagus development

Differentially expressed genes in SOX2 KO vs control forestomach organoids In this dataset, we include expression data from SOX2 KO forestomach organoid and control forestomach organoid In this dataset, we include expression data from SOX2 KO and control forestomach organoids generated from mouse forestomach tissues from Krt5CreER/+; Sox2∆/∆; ROSA26tdTomato/+ mice and wildtype control mice. Organoids were expanded and mechanically dissociated from Matrigel, dispersed using 0.05% Trypsin-EDTA and manual pipetting. RNA was isolated using RNeasy Mini Kit, following the manufacturer’s protocol. For microarray, samples were processed and hybridized to Affymetrix Mouse Gene 2.0 ST by the Washington University Genome Technology Access Core (GTAC). Mouse forestomach organoid GeneChips were analyzed with Transcriptome Analysis Console (Applied Biosystems) analysis software to perform array QC, data normalization, and differential expression analyses.